Figure 16. A: biopsy obtainment; B: cartilage sample (amount 3-4 g with size of rice grains); C: cell culture in white room under GMP conditions; D: after 6-8 weeks the adequate number of cells for implantation are obtained (20-30 million, depending on the size and number of lesions); E: cell seeding in the membrane (biomaterial) in the operating room, at a density of 5 million cells/cm2; F: implantation of the membrane with cells. Membrane suturing.